Cell Cycle Synchronization: Methods and Protocols by Gaspar Banfalvi (auth.), Gaspar Banfalvi (eds.)

By Gaspar Banfalvi (auth.), Gaspar Banfalvi (eds.)

To arrange synchronized cells representing diversified levels of the mobilephone cycle has been an outstanding problem for researchers around the globe. In Cell Cycle Synchronization: equipment and Protocols, specialists within the box provide special protocols delivering first the theoretical history of the technique then step by step directions on tips on how to enforce synchronization in addition to the newest options for the improved examine of regulatory mechanisms to appreciate mobilephone cycle occasions. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian mobilephone cultures, the synchronization equipment provided within the booklet are dependent largely on significant thoughts: the “arrest-and-release” procedure, which contains diversified chemical remedies to dam cells at definite levels of the cellphone cycle, and the actual method, which incorporates actual ways to acquire cells belonging to subpopulations of the mobilephone cycle. Written within the hugely winning Methods in Molecular Biology™ sequence structure, chapters contain introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, conveniently reproducible laboratory protocols, and pointers on troubleshooting and fending off identified pitfalls.

Detailed and simple to stick with, Cell Cycle Synchronization: equipment and Protocols is a perfect consultant for scientists who desire to utilize those robust synchronization innovations yet haven't any entry to thorough, time-tested protocols designed for doing so.

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Sci. 6, 239–242. Kim, J. , and Eidinoff, M. L. (1965) Action of 1-(3-D-arabinofuranosylcytosine on the nucleic acid metabolism and viability of HeLa cells. Cancer Res. 25, 698–702. , Sternberg, S. , and Philips, F. S. (1969) Cytotoxic effects of 1-betaD-arabinofuranosyl-5-fluorocytosine and of 1-beta-D-arabinofuranosylcytosine in proliferating tissues in mice. Cancer Res. 29, 1790–1798. Bertalanffy, F. , and Gibson, M. H. L. (1971) The in vivo effects of arabinosylcytosine on the proliferation of murine B16 melanoma and Ehrlich ascites tumor.

Miller, E. , and Kinsella, T. J. (1992) Radiosensitization by fluorodeoxyuridine: effects of thymidylate synthase inhibition and cell synchronization. Cancer Res. 52, 1687–1694. , and Sigwarth, I. (1978) Comparison of thymidine, 117. 118. 119. 120. 121. 122. 123. 124. 125. 126. 23 fluorodeoxyuridine, hydroxyurea, and methotrexate blocking at the G1 /S phase transition of the cell cycle, studied by replication patterns. Hum. Genet. 45, 193–198. Carl, P. L. (1970) Escherichia coli mutants with temperature-sensitive synthesis of DNA.

The hypothesis that cell cycle synchronization increases transfection efficiency was confirmed in human trabecular meshwork cells. While efficiency of transfection increased about threefold in synchronized cells compared to controls, it still remained low (∼3%) (126). References 1. Watson, J. , and Crick, F. H. (1953) Molecular structure of nucleic acids: a structure for deoxyribonucleic acid. Nature 171, 737–738. 2. , and Pelc, S. (1953) Synthesis of deoxyribonucleic acid in normal and irradiated cells and its relation to chromosome breakage.

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